Review



anti lfa 1α blocking antibody  (Bio X Cell)


Bioz Verified Symbol Bio X Cell is a verified supplier
Bioz Manufacturer Symbol Bio X Cell manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio X Cell anti lfa 1α blocking antibody
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti Lfa 1α Blocking Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/InVivoMAb+anti-mouse+LFA-1%CE%B1/bio_rxiv__2025__11__14__688504-190-7-15
    Average 93 stars, based on 9 article reviews
    anti lfa 1α blocking antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "CD4⁺ T cells confer transplantable rejuvenation via Rivers of telomeres"

    Article Title: CD4⁺ T cells confer transplantable rejuvenation via Rivers of telomeres

    Journal: bioRxiv

    doi: 10.1101/2025.11.14.688504

    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without anti-LFA-1α antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Figure Legend Snippet: ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without anti-LFA-1α antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.

    Techniques Used: In Vivo, Isolation, Flow Cytometry, Activity Assay, Fluorescence, Control, Purification, Transfection, In Vitro, Injection

    Related Articles

    Blocking Assay:

    Article Title: Glia limitans superficialis oxidation and breakdown promote cortical cell death after repetitive head injury
    Article Snippet: .. To block cell adhesion to the endothelium of peripheral immune cells, we used 500 μg anti–LFA-1 (clone M17/4, BE0006) and 500 μg anti–VLA-4 (clone PS/2, BE0071) purchased from BioXcell. .. Rat IgG from BioXcell was used as an isotype control (catalog BE0090).

    Article Title: Glia limitans superficialis oxidation and breakdown promote cortical cell death after repetitive head injury.
    Article Snippet: .. To block cell adhesion to the endothelium of peripheral immune cells, we used 500 μg anti–LFA-1 (clone M17/4, BE0006) and 500 μg anti–VLA-4 (clone PS/2, BE0071) purchased from BioXcell. .. Rat IgG from BioXcell was used as an isotype control (catalog BE0090).

    Article Title: Antibody-mediated B-cell depletion before adoptive immunotherapy with T cells expressing CD20-specific chimeric T-cell receptors facilitates eradication of leukemia in immunocompetent mice
    Article Snippet: The depleting anti-hCD20 antibody 1F5 26 was produced in the FHCRC biologics facility from the hybridoma line HB-9645 obtained from ATCC. .. The blocking anti–LFA-1 (lymphocyte function–associated antigen-1) antibody FD441.8 was obtained from Bio X Cell. .. Flow cytometry was performed with the use of a FACSCanto (BD Biosciences).

    Article Title: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice.
    Article Snippet: Exosome depletion was facilitated by treating Rab27afl/fl Rosa26CreERT2 mice with tamoxifen (2.5mg/mouse/day, Sigma-Aldrich) for 3 days, starting 7 days before hydrodynamic injection. .. Blocking antibodies such as anti-XCL1(50μg/mouse, 80222, R&D Systems), anti-CLEC9A (100ug/mouse, 7H11, BioXCell), anti-ICOS (200μg/mouse, clone 7E.17G9, BioXCell) and anti-LFA-1 (200μg/ mouse, M17/4, BioXCell) were administered i.v. every 3 days, starting 2 days prior to hydrodynamic injection or infection. .. To prevent B-cell re-entry into the splenic white pulp, mice were injected i.v. with antiVLA-4 (200μg/mouse, clone PS/2, BioXCell) and anti-LFA-1 at 200μg per mouse 2 hours as described 54after hydrodynamic injection of pHBV1.3.

    Article Title: The LPS-inactivating enzyme acyloxyacyl hydrolase protects the brain from experimental stroke.
    Article Snippet: Blood-brain-barrier (BBB) disruption is a pathological hallmark of ischemic stroke, and inflammation occurring at the BBB contributes to the pathogenesis of ischemic brain injury.. Lipopolysaccharide (LPS), a cell wall component of Gram-negative bacteria, is elevated in patients with acute stroke.. The activity of LPS is controlled by acyloxyacyl hydrolase (AOAH), a host enzyme that deacylates LPS to inactivated forms.

    Labeling:

    Article Title: Disrupted priming within draining lymph nodes drives immune quiescence in gastric cancer
    Article Snippet: .. Labeled T cells were counted, centrifuged and resuspended in complete T cell media (RPMI+++ containing 50 uM BME and 10 ng/mL IL-2) at 200K/mL, with either isotype control (rat IgG2a kappa, BioXCell) or anti-LFA-1 (M17/4, BioXCell) at a final concentration of 5 ug/mL. .. 500 uL containing 100K T cells were added to each well of a 96 well Polypropylene Deep Well Plate (Corning) into which 5 uL DCs (5,000 DC total) were added.

    Control:

    Article Title: Disrupted priming within draining lymph nodes drives immune quiescence in gastric cancer
    Article Snippet: .. Labeled T cells were counted, centrifuged and resuspended in complete T cell media (RPMI+++ containing 50 uM BME and 10 ng/mL IL-2) at 200K/mL, with either isotype control (rat IgG2a kappa, BioXCell) or anti-LFA-1 (M17/4, BioXCell) at a final concentration of 5 ug/mL. .. 500 uL containing 100K T cells were added to each well of a 96 well Polypropylene Deep Well Plate (Corning) into which 5 uL DCs (5,000 DC total) were added.

    Concentration Assay:

    Article Title: Disrupted priming within draining lymph nodes drives immune quiescence in gastric cancer
    Article Snippet: .. Labeled T cells were counted, centrifuged and resuspended in complete T cell media (RPMI+++ containing 50 uM BME and 10 ng/mL IL-2) at 200K/mL, with either isotype control (rat IgG2a kappa, BioXCell) or anti-LFA-1 (M17/4, BioXCell) at a final concentration of 5 ug/mL. .. 500 uL containing 100K T cells were added to each well of a 96 well Polypropylene Deep Well Plate (Corning) into which 5 uL DCs (5,000 DC total) were added.

    Injection:

    Article Title: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice.
    Article Snippet: Exosome depletion was facilitated by treating Rab27afl/fl Rosa26CreERT2 mice with tamoxifen (2.5mg/mouse/day, Sigma-Aldrich) for 3 days, starting 7 days before hydrodynamic injection. .. Blocking antibodies such as anti-XCL1(50μg/mouse, 80222, R&D Systems), anti-CLEC9A (100ug/mouse, 7H11, BioXCell), anti-ICOS (200μg/mouse, clone 7E.17G9, BioXCell) and anti-LFA-1 (200μg/ mouse, M17/4, BioXCell) were administered i.v. every 3 days, starting 2 days prior to hydrodynamic injection or infection. .. To prevent B-cell re-entry into the splenic white pulp, mice were injected i.v. with antiVLA-4 (200μg/mouse, clone PS/2, BioXCell) and anti-LFA-1 at 200μg per mouse 2 hours as described 54after hydrodynamic injection of pHBV1.3.

    Infection:

    Article Title: Hepatitis B virus surface antigen drives T cell immunity through non-canonical antigen presentation in mice.
    Article Snippet: Exosome depletion was facilitated by treating Rab27afl/fl Rosa26CreERT2 mice with tamoxifen (2.5mg/mouse/day, Sigma-Aldrich) for 3 days, starting 7 days before hydrodynamic injection. .. Blocking antibodies such as anti-XCL1(50μg/mouse, 80222, R&D Systems), anti-CLEC9A (100ug/mouse, 7H11, BioXCell), anti-ICOS (200μg/mouse, clone 7E.17G9, BioXCell) and anti-LFA-1 (200μg/ mouse, M17/4, BioXCell) were administered i.v. every 3 days, starting 2 days prior to hydrodynamic injection or infection. .. To prevent B-cell re-entry into the splenic white pulp, mice were injected i.v. with antiVLA-4 (200μg/mouse, clone PS/2, BioXCell) and anti-LFA-1 at 200μg per mouse 2 hours as described 54after hydrodynamic injection of pHBV1.3.



    Similar Products

    anti active human lfa 1 ab  (ATCC)
    93
    ATCC anti active human lfa 1 ab
    Anti Active Human Lfa 1 Ab, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/KIM127/pmc12702103-233-13-22
    Average 93 stars, based on 1 article reviews
    anti active human lfa 1 ab - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    anti lfa 1α blocking antibody  (Bio X Cell)
    93
    Bio X Cell anti lfa 1α blocking antibody
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti Lfa 1α Blocking Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/InVivoMAb+anti-mouse+LFA-1%CE%B1/bio_rxiv__2025__11__14__688504-190-7-15
    Average 93 stars, based on 1 article reviews
    anti lfa 1α blocking antibody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    anti lfa 1  (Bio X Cell)
    93
    Bio X Cell anti lfa 1
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti Lfa 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/InVivoMAb+anti-mouse+LFA-1%CE%B1/pmc12085615-390-20-24
    Average 93 stars, based on 1 article reviews
    anti lfa 1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    anti-mo lfa-1 beta  (Thermo Fisher)
    90
    Thermo Fisher anti-mo lfa-1 beta
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti Mo Lfa 1 Beta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/anti+lfa+1/pm40229286-327-10-13
    Average 90 stars, based on 1 article reviews
    anti-mo lfa-1 beta - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    anti-lfa-1  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology anti-lfa-1
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti Lfa 1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/lfa+1+antibody/pmc12018561-244-0-3
    Average 90 stars, based on 1 article reviews
    anti-lfa-1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    anti–lfa-1 clone m17/4  (Thermo Fisher)
    90
    Thermo Fisher anti–lfa-1 clone m17/4
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Anti–Lfa 1 Clone M17/4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/anti+lfa+1/med_rxiv__2025__03__17__25324099-229-12-19
    Average 90 stars, based on 1 article reviews
    anti–lfa-1 clone m17/4 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    rabbit anti lfa 1  (Proteintech)
    93
    Proteintech rabbit anti lfa 1
    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without <t>anti-LFA-1α</t> antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.
    Rabbit Anti Lfa 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/CD11a%2FIntegrin+alpha-L+Antibody/pm40024424-103-31-34
    Average 93 stars, based on 1 article reviews
    rabbit anti lfa 1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    invivomab anti mouse lfa 1α cd11a  (Bio X Cell)
    93
    Bio X Cell invivomab anti mouse lfa 1α cd11a
    KEY RESOURCES TABLE
    Invivomab Anti Mouse Lfa 1α Cd11a, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lfa+1/InVivoMAb+anti-mouse+LFA-1%CE%B1/pmc11845304-28-0-5
    Average 93 stars, based on 1 article reviews
    invivomab anti mouse lfa 1α cd11a - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without anti-LFA-1α antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.

    Journal: bioRxiv

    Article Title: CD4⁺ T cells confer transplantable rejuvenation via Rivers of telomeres

    doi: 10.1101/2025.11.14.688504

    Figure Lengend Snippet: ( a ) Experimental design for in vivo River generation with or without CD4⁺ T cell depletion. CD4-proficient or CD4-depleted recipient mice were adoptively transferred with telomere-labelled APCs, pre-loaded or not with Fluad vaccine as antigen source, with or without anti-LFA-1α antibody. Serum was collected 15 days later for River collection. Three months mice were used. ( b ) Representative FACS plots showing efficiency of CD4⁺ T cell depletion (top) and detection of telomere Rivers in each experimental group (bottom). Data are from ( n = 5 mice per group). ( c ) Rejuvenating effects of telomere Rivers. Rivers were isolated from mouse serum as in a by FAVS sorting (PKH67⁺TelC⁺GAPDH⁻) and transplanted subcutaneously into 20-month-old congenic recipients. Animals were bred normally and assessed 12 months later. Flow cytometry of different organs demonstrated reduced senescence markers (β-Gal activity) and increased telomere length. For p16, IL-6, sestrin2-p-p38 (sMAC)), Extended Fig. 10) Data are from ( n = 5 mice per group). MFI, mean fluorescence intensity. Young (3-month-old) mice are shown, as control. ( d ) Artificial River generation, experimental design. APCs were purified from total splenocytes of 3-month-old mice and transfected with siRNAs to silence GAPDH or with control siRNA. APC telomeres were then labelled with TelC probe and ionomycin stimulated in vitro to produce vesicles. Telomere vesicles (siCtrl vesicles) or artificial Rivers (siGAPDH vesicles) were sorted and injected into 20-month-old mice to test their rejuvenating effect. ( e ) Elongation of tissue telomeres by artificial Rivers. Telomere length was evaluated by Flow-FISH in multiple target tissues from the same 20-month-old mice as in d . Results are shown as absolute base-pairs (bp). Data are pooled from ( n = 12 organs per group) and expressed as ΔTL relative to the old baseline at the start of the experiment (T0; grey bar), thereby distinguishing anti-aging effects (maintenance of old baseline; left) from rejuvenating effects (progression toward young reference values; right). siCTRL telomere vesicles largely delayed further telomere attrition without substantial rejuvenation, whereas artificial Rivers (siGAPDH) induced marked telomere elongation toward young reference values. Young (3-month-old) reference telomere lengths are indicated in yellow. In parallel, untreated old mice were aged normally to derive telomere attrition trajectories. ( f ) Appearance of representative mice injected with telomere vesicles (siCtrl vesicles) or with artificial telomere Rivers (siGAPDH vesicles). Note fur and body lean differences ( n = 10 mice per group). One-way Anova for repeated measures with Bonferroni post-test correction ( b - e ). **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars indicate s.e.m.

    Article Snippet: Where indicated, cells were additionally treated with anti-LFA-1α blocking antibody (InVivoMAb anti-mouse CD11a, clone M17/4; Bio X Cell, 1 μg per 106 cells) immediately prior to transfer.

    Techniques: In Vivo, Isolation, Flow Cytometry, Activity Assay, Fluorescence, Control, Purification, Transfection, In Vitro, Injection

    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Inflammation switches the chemoattractant requirements for naive lymphocyte entry into lymph nodes

    doi: 10.1016/j.cell.2024.11.031

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: InVivoMab anti-mouse LFA-1α (CD11a) , Bio X Cell , Cat# BE0006; RRID:AB_1107578.

    Techniques: Control, Virus, Recombinant, Adjuvant, Reverse Transcription, RNAscope, SYBR Green Assay, Plasmid Preparation, Software